CSIR | GATE | NEET | Medical | Life Sciences | XL | Mitosis | Molecular Biology | Caffeine | Hydroxyurea | DNA

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# Experiment: Incompletely replicated DNA blocks onset of mitosis. 

In the experiments schematized here - mammalian cells in culture were treated with caffeine and hydroxyurea, either alone or in combination. 

1. Hydroxyurea blocks DNA synthesis, arresting the cells in S phase and delaying mitosis. 

2. But if caffeine is added as well as hydroxyurea, (i.e. caffeine + hydroxyurea), the delaying mechanism fails, and the cells proceed into mitosis according to their normal schedule with incompletely replicated DNA. 



source: Albert Bruce-Molecular Biology of Cell


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